Hyperosmotic stress-induced somatic embryogenesis and its continuous culture in Japanese honewort (<i>Cryptotaenia japonica</i>)

Japanese honewort (Cryptotaenia japonica) is consumed as a traditional vegetable and has medicinal applications. In Japan, C. japonica mainly produced using hydroponic culture systems; however, damping-off often caused by the adherence of pathogens to its seeds. Therefore, use sterile artificial seeds in likely be effective for preventing disease. this study, we established methods stress-induced somatic embryogenesis seed production honewort. Shoot apex explants from seedlings were treated with 0.7 M sucrose hyperosmotic stress 3 or 6 weeks, then transferred stress-free conditions. Somatic embryos formed after conditions 7 weeks. Stress-treated shoot that cultured Murashige Skoog liquid medium shaking. After 2 weeks culture, approximately 800 each explant. continuously during 37 under same Thus, was effectively induced via stress, embryogenic competence maintained stress- phytohormone-free The used produce enveloping whipped alginate gel avoid hypoxic had high germination rate (72%). This system suitable sterile, highly productive